Artículos de revista

Permanent URI for this collectionhttps://repositorio.inprf.gob.mx/handle/123456789/5

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Author: search.filters.author.Antón-Tay, FernandoSubject: search.filters.subject.Mechanism of action

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    In Vitro Stimulation of Protein Kinase C by Melatonin
    (1998) Antón-Tay, Fernando; Ramírez, Gerardo; Martínez, Isabel; Benítez-King, Gloria; Universidad Autonoma Metropolitana - Iztapalapa, Dpto. de Biología de la Reproducción CBS.; fat@xanum.uam.mx
    It has been shown that melatonin through binding to calmodulin acts both in vitro and in vivo as a potent calmodulin antagonist. It is known that calmodulin antagonists both bind to the hydrophobic domain of Ca2+ activated calmodulin, and inhibit protein kinase C activity. In this work we explored the effects of melatonin on Ca2+ dependent protein kinase C activity in vitro using both a pure commercial rat brain protein kinase C, and a partially purified enzyme from MDCK and N1E-115 cell homogenates. The results showed that melatonin directly activated protein kinase C with a half stimulatory concentration of 1 nM. In addition the hormone augmented by 30% the phorbol ester stimulated protein kinase C activity and increased [3H] PDBu binding to the kinase. In contrast, calmodulin antagonists (500 _M) and protein kinase C inhibitors (100 _M) abolished the enzyme activity. Melatonin analogs tested were ineffective in increasing either protein kinase C activity or [3H] PDBu binding. Moreover, the hormone stimulated protein kinase C autophosphorylation directly and in the presence of phorbol ester and phosphatidylserine. The results show that besides the melatonin binding to calmodulin, the hormone also interacts with protein kinase C only in the presence of Ca2+. They also suggest that the melatonin mechanism of action may involve interactions with other intracellular hydrophobic and Ca2+ dependent proteins.
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    In vitro inhibition of Ca2+/calmodulin-dependent kinase II activity by melatonin
    (1996) Benítez-King, Gloria; Ríos, Amelia; Martínez, Aída; Antón-Tay, Fernando; Instituto Mexicano de Psiquiatría, Departamento de Neurofarmacología, Mexico City, Mexico
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    Characterization of indorenate effects on brain monoamine metabolism
    (1991) Benítez-King, Gloria; Antón-Tay, Fernando; Departmento de Neurofarmacología, Instituto Mexicano de Psiquiatría Mexico