Herpes murine model as a biological assay to test dialyzable leukocyte extracts activity

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dc.contributor.affiliationUnidad de Desarrollo e Investigación en Bioprocesos (UDIBI), Escuela Nacional de Ciencias Biológicas, IPN, Prolongación de Carpio y Plan de Ayala s/n, Col. Sto. Tomás, 11340 México, DF, Mexico.
dc.contributor.emailmarcovelasco@unam.mx (Marco Antonio Velasco-Velazquez) ; smpt.2011@hotmail.com (and Sonia Mayra Perez-Tapia)
dc.creatorSalinas-Jazmín, Nohemíes_ES
dc.creatorEstrada-Parra, Sergioes_ES
dc.creatorBecerril-García, Miguel Angeles_ES
dc.creatorLimón-Flores, Alberto Yairhes_ES
dc.creatorVázquez-Leyva, Saides_ES
dc.creatorMedina-Rivero, Emilioes_ES
dc.creatorPavón, Lenines_ES
dc.creatorVelasco-Velázquez, Marco Antonioes_ES
dc.creatorPérez-Tapia, Sonia Mayraes_ES
dc.date2015
dc.date.accessioned2025-09-10T19:34:52Z
dc.date.accessioned2026-03-27T15:32:36Z
dc.date.available2025-09-10T19:34:52Z
dc.date.issued2015
dc.date.published2015
dc.descriptionHuman dialyzable leukocyte extracts (DLEs) are heterogeneous mixtures of low-molecular-weight peptides that are released on disruption of peripheral blood leukocytes from healthy donors. DLEs improve clinical responses in infections, allergies, cancer, and immunodeficiencies. Transferon is a human DLE that has been registered as a hemoderivate by Mexican health authorities and commercialized nationally. To develop an animal model that could be used routinely as a quality control assay for Transferon, we standardized and validated a murine model of cutaneous HSV-1 infection. Using this model, we evaluated the activity of 27 Transferon batches. All batches improved the survival of HSV-1-infected mice, wherein average survival rose from 20.9% in control mice to 59.6% in Transferon-treated mice. The activity of Transferon correlated with increased serum levels of IFN-γ and reduced IL-6 and TNF-α concentrations. Our results demonstrate that (i) this mouse model of cutaneous herpes can be used to examine the activity of DLEs, such as Transferon; (ii) the assay can be used as a routine test for batch release; (iii) Transferon is produced with high homogeneity between batches; (iv) Transferon does not have direct virucidal, cytoprotective, or antireplicative effects; and (v) the protective effect of Transferon in vivo correlates with changes in serum cytokines.es_ES
dc.formatPDFes_ES
dc.identifier.eissn2314-7156
dc.identifier.issn2314-8861
dc.identifier.urihttps://doi.org/10.1155/2015/146305
dc.identifier.urihttps://repositorio.inprf.gob.mx/handle/123456789/8410
dc.language.isoenges_ES
dc.publisherWileyes_ES
dc.relation2015:146305
dc.relation.jnabreviadoJ IMMUNOL RES
dc.relation.journalJournal of Immunology Research
dc.rightsAcceso Cerradoes_ES
dc.titleHerpes murine model as a biological assay to test dialyzable leukocyte extracts activityes_ES
dc.typeArtículoes_ES

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