Stage-specific modulation of neprilysin and aminopeptidase N in the limbic system during kindling progression

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dc.contributor.affiliationDiv. Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñíz, México D.F., México.es_ES
dc.contributor.emailjoseph@ibt.unam.mxes_ES
dc.creatorDe Gortari, Patricia
dc.creatorVargas, Miguel Ángel
dc.creatorMartínez, Adrián
dc.creatorGarcía-Vázquez, Arlene I.
dc.creatorUribe, Rosa María
dc.creatorChávez-Gutiérrez, Lucia
dc.creatorMagdaleno, Víctor
dc.creatorBoileau, Guy
dc.creatorJean-Louis, Charlí
dc.creatorJoseph-Bravo, Patricia
dc.creator.identificador"GOGP580212MDFRLT06">De Gortari Gallardo, Patricia Bertha Judithes_ES
dc.date.accessioned2017-06-30T01:35:04Z
dc.date.accessioned2026-03-27T14:47:00Z
dc.date.available2017-06-30T01:35:04Z
dc.date.issued2007es_ES
dc.date.published2007es_ES
dc.description.abstractotrodiomaAminopeptidase N (APN) and neprilysin (NEP) inactivate neuropeptides released into the brain extracellular fluid. We previously showed that the expression of pyroglutamyl peptidase II (PPII), the TRH degrading ecto-enzyme, is regulated in rat brain by amygdaline kindling, a paradigm that activates neuronal pathways in the limbic system increasing the expression of several neuropeptides including TRH and opioids. To understand the specificity of this phenomenon, we studied APN and NEP expression in brains of partially or fully kindled rats (stage II and V), sacrificed 6 h after last stimulus, compared with sham-operated animals. In situ hybridization analysis of NEP mRNA levels showed decreased expression at stage II in CA1, CA2, olfactory tubercle and medial mammillary nucleus, and increased at stage V in CA1 and CA2 cells. These changes were specific for the ipsilateral side. APN mRNA levels, semi-quantified by RT-PCR, were decreased at stage II and increased at stage V, in frontal cortex-olfactory tubercle, and hippocampus. NEP and APN enzymatic activities, determined by fluorometric assays, followed similar variations to their respective mRNA levels. The coordinated changes (in some regions) of NEP and APN expression were opposite to those previously observed for PPII mRNA and activity levels in limbic regions. These results demonstrate that expression of ectopeptidases can be regulated when peptide neurons are activated and, that regulation is enzyme-, region-, and stage-specifices_ES
dc.description.monthNoves_ES
dc.identifier1472es_ES
dc.identifier.citationJuan Carlos Bautista Ramírezes_ES
dc.identifier.doi10.1007/s12031-007-0020-9es_ES
dc.identifier.eissn1559-1166es_ES
dc.identifier.issn0895-8696es_ES
dc.identifier.numero3es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.paginacion252-261es_ES
dc.identifier.placeEstados Unidoses_ES
dc.identifier.urihttps://doi.org/10.1007/s12031-007-0020-9es_ES
dc.identifier.urihttps://repositorio.inprf.gob.mx/handle/123456789/6153
dc.identifier.volumen33es_ES
dc.language.isoenges_ES
dc.relation33 (3) 252-261 p.es_ES
dc.relationversión del editores_ES
dc.relation.jnabreviadoJ MOL NEUROSCIes_ES
dc.relation.journalJournal of Molecular Neurosciencees_ES
dc.rightsacceso cerradoes_ES
dc.subject.koNeurologices_ES
dc.subject.koAnimalses_ES
dc.subject.koAntigenses_ES
dc.subject.koGeneticses_ES
dc.subject.koMetabolismes_ES
dc.subject.koElectric Stimulationes_ES
dc.subject.koIn Situ Hybridizationes_ES
dc.subject.koKindinges_ES
dc.subject.koPhysiologyes_ES
dc.subject.koLimbic Systemes_ES
dc.subject.koCD13es_ES
dc.subject.koMalees_ES
dc.subject.koNeprilysines_ES
dc.subject.koRatses_ES
dc.subject.koRats, Wistares_ES
dc.titleStage-specific modulation of neprilysin and aminopeptidase N in the limbic system during kindling progressiones_ES
dc.typearticlees_ES

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