Food-Restricted and Dehydrated-Induced Anorexic Rats Present Differential TRH Expression in Anterior and Caudal PVN. Role of Type 2 Deiodinase and Pyroglutamyl Aminopeptidase II

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dc.contributor.affiliationNeurofisiología Molecular (E.A.-S., C.G.-L., P.de.G.)es_ES
dc.contributor.emailgortari@imp.edu.mx.es_ES
dc.creatorAlvarez-Salas, E.
dc.creatorAceves, C.
dc.creatorAnguiano, B.
dc.creatorUribe, R.M.
dc.creatorGarcía-Luna, C.
dc.creatorSánchez, E.
dc.creatorDe Gortari, P.
dc.creator.identificador"GOGP580212MDFRLT06">De Gortari Gallardo, Patricia Bertha Judithes_ES
dc.date.accessioned2017-06-30T03:40:59Z
dc.date.accessioned2026-03-27T14:50:48Z
dc.date.available2017-06-30T03:40:59Z
dc.date.issued2012es_ES
dc.date.published2012es_ES
dc.description.abstractotrodiomaTRH synthesized in hypothalamic paraventricular nucleus (PVN) regulates thyroid axis function and is also implicated in anorexigenic effects. Under energy deficit, animals present decreasedPVNTRH expression and release, low TSH levels, and increased appetite. Dehydration-induced anorexia (DIA) model allows insight into underlying mechanisms of feeding regulation. Animals drinking a 2.5% NaCl solution for 7 d present body weight reduction; despite their negative energy balance, they avoid food and have increased PVN TRH expression and TSH serum levels. These findings support an inhibiting role of PVN TRH in feeding control. We compared TRH expression by in situ hybridization in PVN subdivisions of 7-d dehydrated male rats to those of a pair-fed group (forced food-restricted) with similar metabolic changes than DIA, but motivated to eat, and to controls.We measured peripheral deiodinase activities, and expression and activity of medial basal hypothalamic type 2 deiodinaseandpyroglutamyl-aminopeptidase II, to understand their regulating role inPVNTRH changes between food restriction and anorexia. TRH mRNA levels increased in anterior (aPVN) and medial-caudal subdivisions in DIA rats, whereas it decreased in medial PVN in both experimental groups. We confirmed the nonhypophysiotropic nature of aPVN TRHergic cells by injecting ip fluorogold tracer. Findings support a subspecialization of TRHergic hypophysiotrophic cells that responded differently between anorexic and food-restricted animals; also, that aPVN TRH participates in food intake regulation. Increased type 2 deiodinase activity seemed responsible for low medial PVN TRH synthesis,whereasincreasedmedialbasalhypothalamicpyroglutamyl-aminopeptidaseII activity inDIA rats might counteract their high TRH releasees_ES
dc.description.monthAgoes_ES
dc.identifier2075es_ES
dc.identifier.citationMaría Teresa Cordero Ortízes_ES
dc.identifier.doi10.1210/en.2011-2011es_ES
dc.identifier.eissn1945-7170es_ES
dc.identifier.issn0013-7227es_ES
dc.identifier.numero8es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.paginacion4067–4076es_ES
dc.identifier.placeUnited Stateses_ES
dc.identifier.urihttps://doi.org/10.1210/en.2011-2011es_ES
dc.identifier.urihttps://repositorio.inprf.gob.mx/handle/123456789/6739
dc.identifier.volumen153es_ES
dc.language.isoenges_ES
dc.publisherThe Endocrine Societyes_ES
dc.relation153 (8) 4067–4076 p.es_ES
dc.relationversión del editores_ES
dc.relation.jnabreviadoENDOCRINOLOGYes_ES
dc.relation.journalEndocrinologyes_ES
dc.rightsacceso cerradoes_ES
dc.subject.koDeiodinase and Pyroglutamyl Aminopeptidase IIes_ES
dc.subject.koRatses_ES
dc.subject.koFood-Restrictedes_ES
dc.subject.koAnorexiaes_ES
dc.subject.kwRatases_ES
dc.subject.kwAlimentos restringidoses_ES
dc.subject.kwAnorexiaes_ES
dc.subject.kwDeiodinase y Piroglutamil Aminopeptidasa IIes_ES
dc.subject.meshmAminopeptidases-Geneticses_ES
dc.subject.meshmAminopeptidases-Metabolismes_ES
dc.subject.meshmAnimalses_ES
dc.subject.meshmAnorexia-Etiologyes_ES
dc.subject.meshmAnorexia-Metabolismes_ES
dc.subject.meshmBody Weight-Physiologyes_ES
dc.subject.meshmCaloric Restrictiones_ES
dc.subject.meshmDehydrationes_ES
dc.subject.meshmEating-Physiologyes_ES
dc.subject.meshmIn Situ Hybridizationes_ES
dc.subject.meshmIodide Peroxidase-Geneticses_ES
dc.subject.meshmIodide Peroxidase-Metabolismes_ES
dc.subject.meshmMalees_ES
dc.subject.meshmParaventricular Hypothalamic Nucleus-Metabolismes_ES
dc.subject.meshmPyrrolidonecarboxylic Acid-Analogs & derivativeses_ES
dc.subject.meshmPyrrolidonecarboxylic Acid-Metabolismes_ES
dc.subject.meshmRatses_ES
dc.subject.meshmReverse Transcriptase Polymerase Chain Reactiones_ES
dc.subject.meshmThyrotropin-Releasing Hormone-Geneticses_ES
dc.subject.meshmThyrotropin-Releasing Hormone-Metabolismes_ES
dc.titleFood-Restricted and Dehydrated-Induced Anorexic Rats Present Differential TRH Expression in Anterior and Caudal PVN. Role of Type 2 Deiodinase and Pyroglutamyl Aminopeptidase IIes_ES
dc.typearticlees_ES

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