Interactions between hormone-mediated and vaccine-mediated immunotherapy for pulmonary tuberculosis in BALB/c mice

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dc.contributor.affiliationExperimental Pathology Laboratory, Department of Pathology, Instituto Nacional de la Nutricion 'Salvador Zubiran', Mexico City, Mexico.es_ES
dc.creatorHernández-Pando, R.
dc.creatorPavón, L.
dc.creatorOrozco, E.H.
dc.creatorRangel, J.
dc.creatorRook, G.A.W.
dc.creator.identificador"HEPR560304HDFRNG06">Hernández Pando, Rogelioes_ES
dc.date.accessioned2017-06-29T04:23:54Z
dc.date.accessioned2026-03-27T14:35:25Z
dc.date.available2017-06-29T04:23:54Z
dc.date.issued2000es_ES
dc.date.published2000es_ES
dc.description.abstractotrodiomaProblems of logistics, compliance and drug resistance point to an urgent need for immunotherapeutic strategies capable of shortening the current 6-month chemotherapy regimens used to treat tuberculosis, or of supplementing ineffective therapy. In this study we sought to define the mechanism of action of two immunotherapies, both of which have previously been shown to prolong survival. Secondly, we wished to identify any clinically useful synergy between these therapies. In BALB-c mice infected via the trachea with Mycobacterium tuberculosis H37Rv there is an initial phase of partial resistance dominated by type 1 cytokines plus tumour necrosis factor-_ (TNF-_) and interleukin-1 (IL-1), followed by a phase of progressive disease. This progressive phase is accompanied by increasing expression of IL-4, and diminished expression of IL-1 and TNF-_. Animals in this late progressive phase of the disease (day 60) were treated with two injections (day 60 and day 90) of 0•1 or 1•0 mg of heat-killed Mycobacterium vaccae, or with 3_,17_-androstenediol (AED; 25 µg subcutaneously three times-week), or with both therapies. We show here using four techniques in parallel (morphometry, immunohistochemistry with automated cell counting, semiquantitative reverse transcription–polymerase chain reaction and enzyme-linked immunosorbent assays of cytokines in lung extracts) that treatment with M. vaccae causes a switch back towards a type 1 cytokine profile, restoration of expression of IL-1_ and TNF-_, and a switch from pneumonia to granuloma. This is very similar to the changes previously seen after treatment with AED. However, there was no evidence for synergy between M. vaccae and AEDes_ES
dc.description.monthJules_ES
dc.identifier306es_ES
dc.identifier.citationJuan Carlos Bautista Ramírezes_ES
dc.identifier.doi10.1046/j.1365-2567.2000.00054.xes_ES
dc.identifier.eissn1365-2567es_ES
dc.identifier.issn0019-2805es_ES
dc.identifier.numero3es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.paginacion391-398es_ES
dc.identifier.placeInglaterraes_ES
dc.identifier.urihttps://doi.org/10.1046/j.1365-2567.2000.00054.xes_ES
dc.identifier.urihttps://repositorio.inprf.gob.mx/handle/123456789/5000
dc.identifier.volumen100es_ES
dc.language.isoenges_ES
dc.relation100 (3) 391-398 p.es_ES
dc.relationversión del editores_ES
dc.relation.jnabreviadoIMMUNOLOGYes_ES
dc.relation.journalImmunologyes_ES
dc.rightsacceso cerradoes_ES
dc.subject.meshAndrostenediol-Therapeutic usees_ES
dc.subject.meshAnimalses_ES
dc.subject.meshAntigens, Bacterial-Immunologyes_ES
dc.subject.meshColony Count, Microbiales_ES
dc.subject.meshCytokines-Biosynthesises_ES
dc.subject.meshEnzyme-Linked Immunosorbent Assayes_ES
dc.subject.meshHypersensitivity, Delayed-Immunologyes_ES
dc.subject.meshImmunotherapy-Methodses_ES
dc.subject.meshImmunotherapy, Activees_ES
dc.subject.meshLung-Immunologyes_ES
dc.subject.meshLung-Microbiologyes_ES
dc.subject.meshMalees_ES
dc.subject.meshMicees_ES
dc.subject.meshMice, Inbred BALB Ces_ES
dc.subject.meshMycobacterium tuberculosis-Immunologyes_ES
dc.subject.meshMycobacterium tuberculosis-Isolation & purificationes_ES
dc.subject.meshReverse Transcriptase Polymerase Chain Reactiones_ES
dc.subject.meshTuberculosis, Pulmonary-Immunologyes_ES
dc.subject.meshTuberculosis, Pulmonary-Pathologyes_ES
dc.subject.meshTuberculosis, Pulmonary-Therapyes_ES
dc.subject.meshAntigens, Bacteriales_ES
dc.subject.meshCytokineses_ES
dc.subject.meshAndrostenedioles_ES
dc.titleInteractions between hormone-mediated and vaccine-mediated immunotherapy for pulmonary tuberculosis in BALB/c micees_ES
dc.typearticlees_ES

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