Browsing by Author "Morales-Mulia, Sandra"

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    Orexin-A promotes EEG changes but fails to induce anxiety in rats
    (Elsevier, 2019) Magdaleno-Madrigal, Víctor Manuel; Morales-Mulia, Sandra; Nicolini, Humberto; Genis-Mendoza, Alma; Cázares-Martínez, Claudia Elizabeth; Pérez-Luna, José Manuel; Morales-Mulia, Marcela; Neurofisiología del Control y la Regulación, Dirección de Neurociencias, INPRFM, México D.F., Mexico; mmulia@imp.edu.mx (M. Morales-Mulia).
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    Orexin-A up-regulates dopamine D2 receptor and mRNA in the nucleus accumbens Shell
    (Springer, 2020) Morales-Mulia, Sandra; Magdaleno-Madrigal, Víctor Manuel; Nicolini, Humberto; Genis-Mendoza, Alma; Morales-Mulia, Marcela; Departamento de Biología Celular, Facultad de Ciencias, UNAM, México, DF, Mexico; mmulia@imp.edu.mx (Marcela Morales-Mulia)
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    ROCK-regulated cytoskeletal dynamics participate in the inhibitory effect of melatonin on cancer cell migration
    (2009) Ortíz-López, Leonardo; Morales-Mulia, Sandra; Ramírez-Rodríguez, Gerardo; Benítez-King, Gloria
    Cell movement is generated by a driving force provided by dynamic cytoskeletal organization. Two main cytoskeletal-dependent features, essential for migration, are the highly cell polarized structure and focal adhesion complexes. Cell migration and substrate anchorage are finely regulated by external signaling exerted by growth factors and hormones. In particular, the serine threonine kinase activated by the small GTPase Rho, the Rho-associated protein kinase (ROCK), participate in both processes through regulation of actin rearrangements in lamellipodia, filopodia, ruffles, and stress fibers. Melatonin, the main product secreted by the pineal gland has oncostatic properties. In MCF-7 cells, 1 nm melatonin reduces migration and invasiveness through increased expression of two cell surface adhesion proteins, E-cadherin and _1-integrin. In this work, we studied the microfilament and microtubule rearrangements elicited by melatonin in migrating leader MCF-7 cells by a wound-healing assay. Additionally, cell anchorage was estimated by quantification of focal adhesions in MCF-7 cells cultured with melatonin. ROCK participation in the indole effects on anchorage and migration was explored by inhibition of the kinase activity with the specific inhibitor of ROCK, the Y-27632 compound. The results indicate that ROCK participates in the melatonin inhibitory effects on cell migration by changing cytoskeletal organization of leader MCF-7 cells. Also, they indicated that indole increased the number of focal contacts through ROCK. These results support the notion that melatonin inhibits cancer cell invasion and metastasis formation via ROCK-regulated microfilament and microtubule organization that converge in a migration/anchorage switch.