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Endomorphin 1 and endomorphin 2 suppress in vitro antibody formation at ultra-low concentrations: anti-peptide antibodies but not opioid antagonists block the activity

dc.creatorAntón, Benito
dc.creatorLeff, Phillipe
dc.creatorCalva, Juan C.
dc.creatorAcevedo, Rodolfo
dc.creatorSalazar, Alberto
dc.creatorMatus, Maura
dc.creatorPavón, Lenin
dc.creatorMartínez, Martín
dc.creatorMeissler, Joseph J.
dc.creatorAdler, Martín W.
dc.creatorGaughan, John P.
dc.creatorEisenstein, Toby K.
dc.date.accessioned2017-06-29T06:02:37Z
dc.date.available2017-06-29T06:02:37Z
dc.date.issued2008es_ES
dc.identifier552es_ES
dc.identifier.issn0889-1591es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/5239
dc.identifier.urihttps://doi.org/10.1016/j.bbi.2008.02.004es_ES
dc.language.isoenges_ES
dc.relation22 (6) 824-832 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.subject.meshAnalgesics, Opioid-immunologyes_ES
dc.subject.meshAnalgesics, Opioid-pharmacologyes_ES
dc.subject.meshAnimalses_ES
dc.subject.meshAntibodies-immunologyes_ES
dc.subject.meshAntibodies-pharmacologyes_ES
dc.subject.meshAntibody Formation-drug effectses_ES
dc.subject.meshChromatography, Affinity-methodses_ES
dc.subject.meshDose-Response Relationship, Druges_ES
dc.subject.meshEnzyme-Linked Immunosorbent Assayes_ES
dc.subject.meshFemalees_ES
dc.subject.meshImmunosuppressive Agents-pharmacologyes_ES
dc.subject.meshMalees_ES
dc.subject.meshMicees_ES
dc.subject.meshMice, Inbred C3Hes_ES
dc.subject.meshNaloxone-pharmacologyes_ES
dc.subject.meshNarcotic Antagonists-pharmacologyes_ES
dc.subject.meshOligopeptides-immunologyes_ES
dc.subject.meshOligopeptides-pharmacologyes_ES
dc.subject.meshOpioid Peptides-immunologyes_ES
dc.subject.meshRabbitses_ES
dc.subject.meshReceptors, Opioid, mu-agonistses_ES
dc.subject.meshReceptors, Opioid, mu-antagonists & inhibitorses_ES
dc.subject.meshSpleen-cytologyes_ES
dc.subject.meshSpleen-drug effectses_ES
dc.subject.meshSpleen-metabolismes_ES
dc.titleEndomorphin 1 and endomorphin 2 suppress in vitro antibody formation at ultra-low concentrations: anti-peptide antibodies but not opioid antagonists block the activityes_ES
dc.typearticlees_ES
dc.contributor.affiliationMolecular Neurobiology and Addictive Neurochemistry Laboratory, National Institute of Psychiatry, Mexico City, Mexico.es_ES
dc.relation.jnabreviadoBRAIN BEHAV IMMUNes_ES
dc.relation.journalBrain Behavior and Immunityes_ES
dc.date.published2008es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.eissn1090-2139es_ES
dc.identifier.doidx.doi.org/10.1016/j.bbi.2008.02.004es_ES
dc.description.monthAgoes_ES
dc.description.abstractotrodiomaEndomorphin 1 (EM-1) and endomorphin 2 (EM-2) were tested for their capacity to alter immune function. Addition of either of these peptides to murine spleen cells in vitro inhibited antibody formation to sheep red blood cells in a bi-phasic dose dependent manner. Maximal inhibition was achieved at doses in the range of 10(-13) to 10(-15)M. Neither naloxone (general opioid receptor antagonist) nor CTAP (selective mu opioid receptor antagonist) blocked the immunosuppressive effect. To show that there was specificity to the immunosuppressive activity of the peptides, affinity-purified rabbit antibodies were raised against each of the synthetic EM peptides haptenized to KLH and tested for capacity to inhibit immunosuppression. Antibody responses were monitored by a standard solid phase antibody capture ELISA, and antibodies were purified by immunochromatography using the synthetic peptides coupled to a Sepharose 6B resin. Verification of the specificity of affinity-purified antisera was performed by immunodot-blot and solid-phase RIA assays. The antisera specific for both EM-1 and EM-2 neutralized the immunosuppressive effects of their respective peptides in a dose-related manner. Control normal rabbit IgG had no blocking activity on either EM-1 or EM-2. These studies show that the endomorphins are immunomodulatory at ultra-low concentrations, but the data do not support a mechanism involving the mu-opioid receptor.es_ES
dc.subject.koEndomorphines_ES
dc.subject.koImmunosuppressiones_ES
dc.subject.koMousees_ES
dc.subject.koPlaque-forming cell assayes_ES
dc.subject.koAnti-endomorphin antibodieses_ES
dc.subject.koNeutralizing antibodieses_ES
dc.subject.koRIAes_ES


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