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dc.creatorLeon-Pinzon, Carolina
dc.creatorCercós, Montserrat G.
dc.creatorNoguez, Paula
dc.creatorTrueta, Citlali
dc.creatorF. De-Miguel, Francisco
dc.date.accessioned2017-06-29T03:49:00Z
dc.date.available2017-06-29T03:49:00Z
dc.date.issued2014es_ES
dc.identifier2708es_ES
dc.identifier.issn1662-5102es_ES
dc.identifier.urihttp://repositorio.inprf.gob.mx/handle/123456789/4557
dc.identifier.urihttps://doi.org/10.3389/fncel.2014.00169es_ES
dc.language.isoenges_ES
dc.publisherLausanne, Switzerland : Frontiers Research Foundationes_ES
dc.relation8 (169) 1-17 p.es_ES
dc.relationversión del editores_ES
dc.rightsacceso cerradoes_ES
dc.titleExocytosis of serotonin from the neuronal soma is sustained by a serotonin and calcium-dependent feedback loopes_ES
dc.typeartículoes_ES
dc.contributor.affiliationInstituto de Fisiología Celular-Neurociencias, Universidad Nacional Autónoma de México es_ES
dc.contributor.emailffernand@ifc.unam.mxes_ES
dc.relation.jnabreviadoFRONT CELL NEUROSCIes_ES
dc.relation.journalFrontiers in Cellular Neuroscience es_ES
dc.identifier.placeSuizaes_ES
dc.date.published2014es_ES
dc.identifier.organizacionInstituto Nacional de Psiquiatría Ramón de la Fuente Muñizes_ES
dc.identifier.doi10.3389/fncel.2014.00169es_ES
dc.description.monthJunes_ES
dc.description.abstractotrodiomaThe soma of many neurons releases large amounts of transmitter molecules through an exocytosis process that continues for hundreds of seconds after the end of the triggering stimulus. Transmitters released in this way modulate the activity of neurons, glia and blood vessels over vast volumes of the nervous system. Here we studied how somatic exocytosis is maintained for such long periods in the absence of electrical stimulation and transmembrane Ca2+ entry. Somatic exocytosis of serotonin from dense core vesicles could be triggered by a train of 10 action potentials at 20 Hz in Retzius neurons of the leech. However, the same number of action potentials produced at 1 Hz failed to evoke any exocytosis. The 20-Hz train evoked exocytosis through a sequence of intracellular Ca2+ transients, with each transient having a different origin, timing and intracellular distribution. Upon electrical stimulation, transmembrane Ca2+ entry through L-type channels activated Ca2+-induced Ca2+ release. A resulting fast Ca2+ transient evoked an early exocytosis of serotonin from sparse vesicles resting close to the plasma membrane. This Ca2+ transient also triggered the transport of distant clusters of vesicles toward the plasma membrane. Upon exocytosis, the released serotonin activated autoreceptors coupled to phospholipase C, which in turn produced an intracellular Ca2+ increase in the submembrane shell. This localized Ca2+ increase evoked new exocytosis as the vesicles in the clusters arrived gradually at the plasma membrane. In this way, the extracellular serotonin elevated the intracellular Ca2+ and this Ca2+ evoked more exocytosis. The resulting positive feedback loop maintained exocytosis for the following hundreds of seconds until the last vesicles in the clusters fused. Since somatic exocytosis displays similar kinetics in neurons releasing different types of transmitters, the data presented here contributes to understand the cellular basis of paracrine neurotransmission.es_ES
dc.subject.koExocytosises_ES
dc.subject.koExtrasynaptices_ES
dc.subject.koSomatic Exocytosises_ES
dc.subject.koExtrasynaptic Releasees_ES
dc.subject.koSerotonines_ES
dc.subject.ko5-HTes_ES
dc.subject.koCalciumes_ES
dc.subject.koPositive Feedbackes_ES


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